In 1975, he began working for the United States Fish and Wildlife Service in Wyoming where he studied pronghorn antelope and sharp-tailed grouse. In 1980 he moved to Alaska where he took over the United States Fish and Wildlife Service's fledgling Polar Bear Research Project. In 1996 Amstrup's research position was transferred to the United States Geological Survey. During his 30-years in Alaska, he studied polar bear ecology, primarily in the Beaufort Sea. In 2007, Amstrup's team of scientists prepared nine reports leading to the 2008 listing, by United States Secretary of the Interior Dirk Kempthorne, of polar bears as a threatened species under the Endangered Species Act. In 2010, he published an article in Nature finding that even if climate change led to complete melting of the polar ice packs, the ice could return if global temperatures subsequently cooled. He has taught at the University of Wyoming as an adjunct professor since 2006.