Holonyak earned his bachelor's (1950), master's (1951), and doctoral (1954) degrees in electrical engineering from the University of Illinois at Urbana-Champaign. Holonyak was John Bardeen's first Ph.D. student there; Bardeen, a theoretical physicist, was the co-inventor of the transistor who ultimately won Nobel Prizes for that work and for the theory of superconductivity. In 1954 Holonyak went to Bell Telephone Laboratories, where he worked on silicon-based electronic devices. From 1955–1957 he served with the U.S. Army Signal Corps. From 1957–1963 he was a scientist at the General Electric Company's Advanced Semiconductor Laboratory in Syracuse, New York. In 1963, he became a professor at the University of Illinois, from which he retired fifty years later in 2013.