On 14 December 2009, Trulli was confirmed as one of the newly formed Lotus team's drivers, joining former McLaren driver Heikki Kovalainen. He only finished one of the opening four races, a weaker reliability record than Kovalainen, leading him to note that "everything happens on my car and my car only – so to this day, my expectations have not been met".
At the launch of the Lotus T127, Trulli admitted in an interview with Autosport that US F1 and Sauber had been in contact with him. In late 2009, Trulli was asked to test a NASCAR stock car in North America for Toyota. The car was set up by Michael Waltrip Racing.