Eisele remained on the crew, and on October 11, 1968, Apollo 7 was launched on an 11-day mission—the first crewed flight test of the third generation United States spacecraft. By this time, the Senior Pilot title was changed to Command Module Pilot. Together with spacecraft commander Schirra and Lunar Module Pilot Cunningham, Eisele performed simulated transposition and docking maneuvers with the upper stage of their Saturn IB launch vehicle, and acted as navigator, taking star sightings and aligning the spacecraft's guidance and navigation platform. The crew completed eight successful test firing maneuvers of the service module's propulsion engine. They also tested the performance of all spacecraft systems and broadcast the first live televised coverage of crew activities.
Apollo 7 was placed in an Earth-orbit with an apogee of 153.5 nautical miles (284.3 km; 176.6 mi) and perigee of 122.6 nautical miles (227.1 km; 141.1 mi). The 260-hour, 4.5 million mile (7.25 Gm; 7.25 million km) shakedown flight was successfully concluded on October 22, 1968, with splashdown occurring in the Atlantic, 8 miles (15 km) from the carrier USS Essex and only 0.3 miles (0.48 km; 480 m) from the predicted target. Eisele logged 260 hours in space.