Born and raised in Chicago, Illinois, DeLong is the daughter of a building contractor father and a homemaker mother. Growing up, DeLong stated that her father had told her to choose a practical career. Before entering the field of criminal profiling, DeLong worked as a psychiatric nurse at Northwestern University Hospital in Chicago, Illinois. However, by the time she was 28 years old, she was a divorcee with a young son. Wanting a new career, DeLong went to Quantico, Virginia, in 1980 to attend the FBI training academy. She was one of only seven female recruits. DeLong worked in the FBI's Chicago office after graduating from Quantico. DeLong stated that she used humor to ease tensions while working. She claimed that her three career goals were as follows: "to be involved with a high-profile national criminal, I wanted to apprehend a serial killer, and I wanted to rescue a kidnap victim alive."