In late November 2006, Cameron was given the Class A Playoff Performer Award by MiLB.com. He began the 2007 season with the Lakeland Flying Tigers of the High-A Florida State League. On August 9, 2007, Maybin was promoted from the Single-A Lakeland Flying Tigers to the Double-A Erie SeaWolves where he played six games before his call up to the Major League.
Maybin was called up to the Tigers on August 17, 2007, and made his Major League debut that day, going 0-for-4. He was the youngest player in the American League (20 years old). In his second game, August 18, 2007, Maybin picked up his first career hit, (a single), and first career home run, both off of Roger Clemens. During his next plate appearance, he was hit by a Clemens fastball. Thus, Maybin received his first Major League hit, home run and hit-by-pitch in the same game.
On December 5, 2007, the Tigers traded Maybin, Andrew Miller, Mike Rabelo, Dallas Trahern, Eulogio De La Cruz, and Burke Badenhop to the Florida Marlins for Dontrelle Willis and Miguel Cabrera.